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प्रश्न
What are the restriction endonucleases? Give the rules of their nomenclature.
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उत्तर
They cut DNA duplex at specific points. They are molecular scissors.
Rules: Restriction endonucleases are named by using the first letter of the name of the genus followed by the first two letters of species from which these enzymes are isólated. All three letters are written in italics.
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संबंधित प्रश्न
Define biotechnology? Which are the basic principles and process of biotechnology?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the CORRECT sequence of steps in PCR.
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
DNA fingerprinting refers to ______.
Name two restriction enzymes used in PCR.
What is polymerase chain reaction (PCR)?
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Importance of PCR.
