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प्रश्न
What are the restriction endonucleases? Give the rules of their nomenclature.
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उत्तर
They cut DNA duplex at specific points. They are molecular scissors.
Rules: Restriction endonucleases are named by using the first letter of the name of the genus followed by the first two letters of species from which these enzymes are isólated. All three letters are written in italics.
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संबंधित प्रश्न
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
What is the cell that receives a recombinant gene called?
What is Recognition sequence? Explain in brief.
The gene transfer between unrelated plants is brought about by ____________.
Lytic cycle involves bacteriophage and ____________.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
What is polymerase chain reaction (PCR)?
In vitro amplification of DNA segments is called______.
