Question

Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:

5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'

1. Name a restriction enzyme which can recognise this DNA sequence.
2. Write the sequence after digestion.
3. Why are the ends generated after digestion called sticky ends?

Answer 1

(i) EcoRI

(ii)

5'CAG3'	5'AATTCTTA3'
3'GTCTTAA 5'	3'GAAT5'

(iii) Because they create hydrogen connections with their complementary cut sections, these are known as sticky ends.