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प्रश्न
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
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उत्तर
(i) EcoRI
(ii)
| 5'CAG3' | 5'AATTCTTA3' |
| 3'GTCTTAA 5' | 3'GAAT5' |
(iii) Because they create hydrogen connections with their complementary cut sections, these are known as sticky ends.
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संबंधित प्रश्न
Distinguish between plasmid DNA and chromosomal DNA.
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Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
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Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
What is true for plasmid?
How many sets of primers are required in each cycle of PCR?
