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प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
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उत्तर
- The Bt toxin is encoded by the cry gene. The cry I AC and Cry II Ab control cotton boll worms, while the cry I Ab controls corn borer. Bt gene produces Bt toxin. This toxin provides resistance to plants against lepidopteron, coleopteron and dipterans pests.
- An example is Bt cotton, in which the gene from the bacterium that encodes for the toxin is incorporated. The activated Bt toxin binds to the surface of the midgut epithelium of the insects and causes swelling and cell lysis, which eventually leads to the death of the insects.
- In the process of producing insect-resistant crops, specific Bt toxin genes are isolated from B.thuringiensis, and incorporated into the crops. Since these toxins are insect specific, they do not harm the crops or humans.
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संबंधित प्रश्न
What is PCR? Explain different steps involved in it.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Match the column I (PCR steps) and Column II (Temperature). Select the correct option.
| Column I (PCR steps) | Column II (Temperature) | ||
| i. | Polymerization | P. | 40 - 60°C |
| ii. | Denaturation | Q. | 70 - 75°C |
| iii. | Primer annealing | R. | 90 - 98°C |
| S. | 28 - 37°C |
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- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
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- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
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- Name the cloning vector shown above. In which organism in this cloning vector inserted?
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Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
