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प्रश्न
What are plasmids?
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उत्तर
Plasmids are small, circular, double-stranded, self-replicating DNA molecules.
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संबंधित प्रश्न
Give one significant difference between:
Electroporation and Gene Gun.
Give the steps in PCR or polymerase chain reaction with suitable diagrams.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
The polymerase chain reaction is a powerful technique to ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
Write steps involved in gene amplification.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
Expand YAC used in the field of Biotechnology.
State the steps involved in the process of gene therapy for the treatment of ADA-deficiency.
