Advertisements
Advertisements
प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist. Who in your opinion is right?
Advertisements
उत्तर
Both. As biotechnology is a very wide area that deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned the gene for the antigen (that is used as a vaccine) in an organism, which allows the production of the antigen in large amounts.
संबंधित प्रश्न
Explain the work carried out by Cohen and Boyer that contributed immensely in biotechnology.
Name and explain the technique used for separating DNA fragments and making them available for biotechnology experiments.
What are the restriction endonucleases? Give the rules of their nomenclature.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
A PCR machine can raise the temperature up to 100°C but after that, it is not able to lower the temperature below 70°C automatically. Which step of PCR will be hampered first in this faulty machine? Explain why?
The enzyme restriction endonuclease ______.
Ti plasmid being used for introducing genes in plants obtained from ______.
What is plasmid?
Define biotechnology? Which are the basic principles and process of biotechnology?
Explain types of enzymes used in biotechnology?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the properties that a cloning vector must possess to be efficiently used in rDNA technology.
- Control elements like promoter, operator, ribosomal binding sites
- Restriction sites
- Marker genes
- Ori of replication
- Ability to replicate independently
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
The gene transfer between unrelated plants is brought about by ____________.
Human insulin is produced by culturing the genetically engineered ______
A hind of biotechnology involving manipulation of DNA is ______.
Nucleic acid segment tagged with a radioactive molecule is called ______.
Name two restriction enzymes used in PCR.
Which of these are most widely used in genetic engineering?
What is polymerase chain reaction (PCR)?
Describe three steps involved in mechanism of PCR.
______technique is used for in vitro gene cloning or gene multiplication.
Write a short note on vectors.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
How does EcoRI differ from an exonuclease?
Explain the mechanism of PCR.
How many sets of primers are required in each cycle of PCR?
Give a reason for the following:
DNA cannot enter directly into the host cell.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
