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प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist. Who in your opinion is right?
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उत्तर
Both. As biotechnology is a very wide area that deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned the gene for the antigen (that is used as a vaccine) in an organism, which allows the production of the antigen in large amounts.
संबंधित प्रश्न
Rearrange the following in the correct sequence to accomplish an important biological reaction:
(a) In vitro synthesis of copies of DNA of interest
(b) Chemically synthesized oligonucleotides
(c) Enzyme DNA-polymerase
(d) Complementary region of DNA
(e) Genomic DNA template
(f) Nucleotides provided
(g) Primers
(h) Thermostable DNA-polymerase (from Thermus aquaticus)
(i) Denaturation of ds-DNA
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
What would be the molar concentration of human DNA in a human cell?
How has the development of bioreactor helped in biotechnology?
The biological scissor is ______.
Give the applications of the PCR technique.
Give one significant difference between:
Electroporation and Gene Gun.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
What is the cell that receives a recombinant gene called?
The enzyme restriction endonuclease ______.
What is Palindromic sequence?
Match the Column I (Restriction enzyme) with Column II (Recognition sequence) and select the correct option.
| column I (Restriction Enzyme) | column II (Recognition sequence) | ||
| i. | Alu | P. | 5'---G- ↓-G-A-T-C-C---3' 3'---C-C-T-A-G- -G---5 |
| ii. | BamHI | Q. | 5'--- A-G- ↓ -C-T---3' 3' ---T-C- G-A--- 5' |
| iii. | Eco RI | R. | 5'--G-T-C- ↓ -G-A-C--3' I 3'--C-A-G-t-C-T-G--5' |
| iv. | HindII | S. | 5'---G- ↓ -A-A-T-T-C---3' 3'---C-T-T-A-A- -G---5' |
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
The gene transfer between unrelated plants is brought about by ____________.
Human insulin is produced by culturing the genetically engineered ______
Which of the following statements is correct with respect to cellular respiration?
Which one is a true statement regarding DNA polymerase used in PCR?
A hind of biotechnology involving manipulation of DNA is ______.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Which of the following enzyme catalyses the removal of nucleotides from the end of DNA molecules?
Which of these are most widely used in genetic engineering?
What is polymerase chain reaction (PCR)?
To be a cloning vector, a plasmid require ______.
Plasmid is ______.
How does EcoRI differ from an exonuclease?
Expand YAC used in the field of Biotechnology.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
When the phage DNA enters the host bacterium, the host cell protects itself from the viral DNA attacks with the help of ______ enzyme.
