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प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist. Who in your opinion is right?
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उत्तर
Both. As biotechnology is a very wide area that deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned the gene for the antigen (that is used as a vaccine) in an organism, which allows the production of the antigen in large amounts.
संबंधित प्रश्न
The biological scissor is ______.
Expand the following abbreviation: BAC
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Why are bio-fertilizers preferred over chemical fertilizers?
Give a reason why :
Proteases are added during the isolation of DNA for genetic engineering.
The bacterium which causes a plant disease called crown gall is _______________.
The technique which involves addition or deletion of genes is ______.
Define vector? write any two examples,
What is gene cloning? Explain different tools used for it.
From the following which one is NOT a feature of the plasmids?
Which of the following is TRUE regarding EcoR I?
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Identify the CORRECT sequence of steps in PCR.
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
Identify the CORRECT order of steps in Polymerase Chain Reaction (PCR).
Human insulin is produced by culturing the genetically engineered ______
A technique used to make numerous copies of a specific DNA segment quickly and accurately is called ______
Which of the following statements is correct with respect to cellular respiration?
Cloning of genes, play a very significant role in genetic engineering, helping the transfer of desirable foreign genes into different hosts. The scientists, to make this process easier and effective are creating engineered vectors in such a way that they help easy linking of foreign DNA and selection of recombinants from non-recombinants. 'pBR322' is one such engineered vectors developed by scientists. A diagram of an engineered vector pBR322 is given below:
- Name the host for this cloning vector.
- Identify 'Rop' and 'Ori' in the diagram from 'U', 'V', 'W', 'X', 'Y' and 'Z'. Write their functions.
- Draw the fragments that will be formed by the action of 'Z' (marked in the diagram) on the specific site of the DNA segment given below:
5' --- GTACGAATCCTGA --- 3'
3' --- CATGCTTAGGACT --- 3'
Expand the following acronym:
BAC
Restriction enzymes were discovered by ______.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Name the enzymes that are called molecular scissors.
In vitro amplification of DNA segments is called______.
David is a molecular biologist. He uses a technique to know the amino acids occupy the first and the last positions in the Chain - A and Chain - B of insulin. Name the scientist whose contribution made it possible for David to use this technique.
