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प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist. Who in your opinion is right?
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उत्तर
Both. As biotechnology is a very wide area that deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned the gene for the antigen (that is used as a vaccine) in an organism, which allows the production of the antigen in large amounts.
संबंधित प्रश्न
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
Enlist and write in brief about the different biological tools required in rDNA technology.
Explain types of enzymes used in biotechnology?
From the following which one is NOT a feature of the plasmids?
For which of the following reason Agrobacterium tumefaciens is most widely used for gene transfer in plants?
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
In which of the following techniques, charged molecules (DNA, RNA and Proteins) are separated by applying the electric field?
____________ cuts the DNA within the specific positions.
______ is the soil bacterium that causes crown gall characterized by tumors in plants.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
Exonuclease performs the function of ____________.
Which one is a true statement regarding DNA polymerase used in PCR?
Which of the following is not required in the preparation of a recombinant DNA molecule?
What is meant by gene cloning?
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
The given figure is the diagrammatic representation of the E. coli vector pBR322. Which one of the given options correctly indentites its certain component(s)?
Describe three steps involved in mechanism of PCR.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
The polymerase chain reaction is a powerful technique to ______.
Plasmid is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
How gene amplification is done?
Write steps involved in gene amplification.
Expand YAC used in the field of Biotechnology.
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
