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प्रश्न
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A wine maker and a molecular biologist who has developed a recombinant vaccine, both claim themselves to be biotechnologist. Who in your opinion is right?
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उत्तर
Both. As biotechnology is a very wide area that deals with techniques of using a ‘natural’ organism (or its parts) as well as genetically modified organisms to produce products and processes useful for mankind. A winemaker employs a strain of yeast to produce wine by fermentation (a natural phenomenon), while the molecular biologist has cloned the gene for the antigen (that is used as a vaccine) in an organism, which allows the production of the antigen in large amounts.
संबंधित प्रश्न
Name the genes responsible for making Bt cotton plants resistant to bollworm attack. How do such plants attain resistance against bollworm attacks? Explain.
What does PCR stand for? Describe the different steps of PCR
Write a short note on Electrophoresis.
In vitro amplification of DNA or RNA segment is known as ___________.
The enzyme restriction endonuclease ______.
What is Recognition sequence? Explain in brief.
Identify the palindromic sequence which is recognized by the EcoRI restriction enzyme.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
The vector Baculovirus is used for gene transfer in ____________.
Lytic cycle involves bacteriophage and ____________.
Exonuclease performs the function of ____________.
Which of the following viruses has the capacity to produce lysozymes?
Nucleic acid segment tagged with a radioactive molecule is called ______.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
What is meant by gene cloning?
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
Which of the following statements are true about cloning vectors?
- Vectors are DNA molecules that carry a foreign DNA segment.
- The replicated DNA is introduced into the host cell.
- It possesses the origin of replication (Ori).
- Vectors may be plasmid, bacteriophage, Yeast artificial chromosomes (YACs), etc.
- Vectors do not require a selectable marker to identify and eliminate non-transformants.
Choose the correct answer from the options given below.
What is polymerase chain reaction (PCR)?
To be a cloning vector, a plasmid require ______.
What is EcoRI?
Which amino acid is coded by the initiation codon?
Expand YAC used in the field of Biotechnology.
Name the source of thermostable DNA polymerase.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
Assertion: In a bioreactor, it is not necessary to maintain sterile ambience.
Reason: Sterile conditions promote the growth of unwanted microbes in the culture medium.
What is the relationship between alien DNA and the origin of replication in the concept of cloning?
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
What is the function of DNA ligase in the creation of recombinant DNA?
