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प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
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उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
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संबंधित प्रश्न
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Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
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Which of the following is NOT involved in genetic engineering technique?
i. Combining genes from two organisms
ii. Gene cloning
iii. Transfer of lipid molecules
iv. Transfer of genes to new organisms
v. Repairing or replacing the defective genes by healthy genes
vi. Artificial synthesis of new gene
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Annealing step of PCR operates at ______ °C.
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
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(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
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What is the importance of PCR.
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