Advertisements
Advertisements
प्रश्न
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
Advertisements
उत्तर
When a DNA molecule is created by ligating a gene to a plasmid vector, It becomes a circular DNA which is ready to replicate in host organism. After this stage, addition of exonuclease is not going to affect the process because the DNA does not have a free end and hence enzyme exonuclease will not get a substrate to show its action. So, in this experiment; bacterial transformation is not going to be disturbed.
APPEARS IN
संबंधित प्रश्न
What would be the molar concentration of human DNA in a human cell?
Give one significant difference between:
Electroporation and Gene Gun.
The technique which involves addition or deletion of genes is ______.
What is plasmid?
What is Palindromic sequence?
Define biotechnology? Which are the basic principles and process of biotechnology?
What is gene cloning? Explain different tools used for it.
From the following list of cloning vectors used in rDNA technology identify the vectors that are the most commonly used.
Plasmids, bacteriophages (Ml3, lambda virus), cosmid, phagemid, BAC (bacterial artificial chromosome), YAC (yeast artificial chromosome), transposons, baculoviruses, and mammalian artificial chromosomes (MACs)
DNA polymerase enzyme used in PCR is isolated from ____________ bacteria.
Which of the following device is required for PCR?
Which of the following is an INCORRECT match?
Lytic cycle involves bacteriophage and ____________.
Which of the following viruses has the capacity to produce lysozymes?
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
Name two restriction enzymes used in PCR.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
Write steps involved in gene amplification.
What is EcoRI?
