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प्रश्न
What is Palindromic sequence?
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उत्तर
The sequences which read same in forward and reverse direction are known as palindromic sequences.
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संबंधित प्रश्न
Give an example of the source of thermostable enzyme DNA polymerase.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Identify the characters that a cloning vector should possess.
i. Origin of replication (Ori)
ii. Ability to destroy the alien DNA
ii. Cloning site to link the alien DNA
iv. The tumour inducing plasmid Ti
v. Selectable marker
vi. Low molecular weight
Which of the following viruses has the capacity to produce lysozymes?
Both a wine maker and a molecular biologist who had developed a recombinant vaccine claim to be biotechnologists. Who in your opinion is correct?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
What would happen when one grows a recombinant bacterium in a bioreactor but forget to add antibiotic to the medium in which the recombinant is growing?
pBR322, used very frequently in cloning experiments as a vector in E. coli is ______.
The structure below shows pUC18, which is similar to pBR322 in its function. However, they differ in some of their restriction sites and the number of ori. The ori number for pBR322 is approximately 20.
- How are puc18 and pBR322 used in biotechnological studies?
OR
What will be the impact if the ori in the above structure gets damaged? - The lac z gene has many recognition sites. Study the segment of DNA given below and answer the questions.
5’... ATC GTA AAG CTT CAT…3’
3’... TAG CAT TTC GAA GTA…5’
i) Applying your knowledge of palindrome sequences identify and mark the possible region where the restriction enzyme X will act.
ii) Restriction enzyme Y was used to extract a gene of interest from a plant. This gene needs to be inserted in the given DNA segment which has been treated with restriction enzyme X. Will there be a successful recombination? Explain with a reason. - Which one of the two (pUC18 and pBR322) would you prefer for biotechnological studies? Justify.
