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प्रश्न
Which of the following is the correct recognition sequence of restriction enzyme Eco RI?
पर्याय
S' _ _ _ A _ G ↓ _ C _ T _ _ _ 3'
3' _ _ _ T _ C _ ↑ _ G _ A _ _ _ S'
5' _ _ _ G _ _ G _ A _ T _ C _ C _ _ _ 3'
3' _ _ _ C _ C _ T _ A _ G _ _ G _ _ _ 5'
5' _ _ G _ T _ C _ ↓ _ G _ A _ C _ _ 3'
3' _ _ C _ A _ G _ ↑ _ C _ T _ G _ _ 5'
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
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उत्तर
5' _ _ _ G _ ↓ _ A _ A _ T _ T _ C _ _ _ 3'
3' _ _ _ C _ T _ T _ A _ A _ ↑ _ G _ _ _ 5'
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संबंधित प्रश्न
State the role of DNA ligase in biotechnology.
Explain the mechanism of action of restriction endonucleases that makes them suitable for genetic engineering.
Explain the properties of a good or ideal cloning vector for rDNA technology.
Which of the following DNA sequence is recognised and cut by EcoR I?
Study the following statements regarding separation of DNA using agarose gel electrophoresis.
- Large fragments of DNA molecules move faster than small DNA fragments and thus separate faster.
- DNA being negatively charged migrates to cathode.
____________ is the manipulation of genetic material towards a desired end, in a directed and predetermined way, using in vitro process.
What is meant by gene cloning?
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Write steps involved in gene amplification.
