Advertisements
Advertisements
प्रश्न
Who among the following was awarded the Nobel Prize for the development of PCR technique?
पर्याय
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
Advertisements
उत्तर
Kary Mullis
APPEARS IN
संबंधित प्रश्न
What does PCR stand for? Describe the different steps of PCR
What are the restriction endonucleases? Give the rules of their nomenclature.
In vitro amplification of DNA or RNA segment is known as ___________.
Explain the properties of a good or ideal cloning vector for rDNA technology.
The technique which involves addition or deletion of genes is ______.
What is Palindromic sequence?
Define vector? write any two examples,
Vectors are ____________ molecules that carry a foreign gene and replicate inside the host cell.
Identify the CORRECT sequence of steps in PCR.
The following identifies the major features of technology that differentiate modem biotechnology from classical biotechnology.
i. Capability of science to change the genetic material for getting new specific products through rDNA technology, polymerase chain reaction (PCR), microarrays, cell culture and fusion, and bioprocessing.
ii. Ownership of technology and its sociopolitical impact.
iii. Modem biotechnology makes use of only fermentation technology.
iv. Classical biotechnology relies only on principles of plant and animal tissue culture.
The vector Baculovirus is used for gene transfer in ____________.
A recombinant DNA molecule was created by ligating a gene to a plasmid vector. By mistake, an exonuclease was added to the tube containing the recombinant DNA. How does this affect the next step in the experiment i.e. bacterial transformation?
| Oil spill is a major environmental issue. It has been found that different strains of Pseudomonas bacteria have genes to break down the four major groups of hydrocarbons in oil. Trials are underway to use different biotechnological tools to incorporate these genes and create a genetically engineered strain of Pseudomonas - a ‘super-bug’, to break down the four major groups of hydrocarbons in oil. Such bacteria might be sprayed onto surfaces polluted with oil to clean thin films of oil. |
(a) List two advantages of using bacteria for such biotechnological studies?
(b) For amplification of the gene of interest PCR was carried out. The PCR was run with the help of polymerase which was functional only at a very low temperature. How will this impact the efficiency of the PCR? Justify.
(c) If such bacteria are sprayed on water bodies with oil spills, how will this have a positive or negative effect on the environment? Discuss.
Identify the desirable characteristics for a plasmid used in rDNA technology from the following.
- Ability to multiply and express outside the host in a bioreactor.
- A highly active promoter.
- A site at which replication can be initiated.
- One or more identifiable marker genes.
- One or more unique restriction sites.
The DNA polymerase enzyme used in PCR is obtained from______.
______technique is used for in vitro gene cloning or gene multiplication.
Study the diagram given below and answer the questions that follow.
- Name the cloning vector shown above. In which organism in this cloning vector inserted?
-
Mention any two restriction sites shown in the diagram.
- Name any two selectable markers shown in the diagram.
The restriction enzymes used as molecular scissors are type of ______.
Who accomplished the creation of the first artificial recombinant DNA molecule, and what was linked together?
