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प्रश्न
Who among the following was awarded the Nobel Prize for the development of PCR technique?
पर्याय
Herbert Boyer
Hargovind Khurana
Kary Mullis
Arthur Kornberg
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उत्तर
Kary Mullis
APPEARS IN
संबंधित प्रश्न
Retroviruses have no DNA. However, the DNA of the infected host cell does possess viral DNA. How is it possible?
Distinguish between RNA and DNA.
Give an example of the source of thermostable enzyme DNA polymerase.
The recognition sequence of restriction enzymes is generally ______ nucleotide long.
Enlist different types of restriction enzymes commonly used in rDNA technology? Write about their role.
ECoRI is obtained from ______.
For which of the following purpose restriction endonuclease enzyme is used in genetic engineering?
Following are the four statements regarding separation of DNA fragments using Gel electrophoresis. Identify the INCORRECT statements.
i. DNA is negatively charged molecule and so it is loaded on gel towards the Anode terminal.
ii. DNA fragments travel along the surface of the gel whose concentration does not affect movement of DNA.
iii. Smaller the size of DNA fragment, larger is the distance it travels through it.
iv. Pure DNA can be visualized directly by exposing to UV radiation.
Polymerase Chain Reaction is made possible by ____________.
In PCR technique, the organism providing thermostable DNA polymerase is a ____________.
The most important feature in a plasmid to serve as a vector in gene cloning experiment is ______.
Observe the given sequence of nitrogenous bases on a DNA fragments and answer the following questions:
5' - CAGAATTCTTA - 3'
3' - GTCTTAAGAAT - 5'
- Name a restriction enzyme which can recognise this DNA sequence.
- Write the sequence after digestion.
- Why are the ends generated after digestion called sticky ends?
The PCR technique is used for ______.
The plasmid of Agrobacterium tumefaciens that is now modified as a cloning vector is ______.
Tissue plasminogen activator and urokinase are used in the treatment of ______.
What is EcoRI?
Name the source of thermostable DNA polymerase.
Give a reason for the following:
DNA cannot enter directly into the host cell.
Suneeta is planning an experiment to clone a gene in a vector. So, she has to choose a good cloning vector.
Which one of the vectors shown below should she choose? Justify your answer by giving two reasons.
 |
 |
 |
| Vector A | Vector B | Vector C |
Give a reason for the following:
The diagram given below represents vector pBr322 which was modified by a research scholar Dr. Rayon, but the vector was rejected by his guide.
