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प्रश्न
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
पर्याय
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
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उत्तर
A continuous culture system
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संबंधित प्रश्न
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Explain briefly:
PCR
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The first step in recombinant DNA technology is
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Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
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- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
State the advantage of using Thermostable DNA polymerase.
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Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
