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प्रश्न
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
पर्याय
Both (A) and (R) are true and (R) is the correct explanation of (A)
Both (A) and (R) are true, but (R) is not the correct explanation of (A)
(A) is true, but (R) is false
(A) is false, but (R) is true
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उत्तर
Both (A) and (R) are true, but (R) is the correct explanation of (A)
Explanation:
In order to base-pair with a strand of DNA or RNA, oligonucleotides are created. Oligonucleotides are most frequently used as polymerase chain reaction primers. The 5' end of the sequence intended for amplification serves as the complement to at least part of the sequence used in the construction of primers. The primers anneal to the complementary sequences on the DNA templates during the annealing process.
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संबंधित प्रश्न
Which of the following term used for defining a viral genome incorporated into host DNA?
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While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
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- Cutting of DNA at specific location by restriction enzyme.
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Choose the correct answer from the options given below:
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
