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प्रश्न
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
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उत्तर
Thermus aquaticus is the scientific name of the organism that produces thermostable DNA polymerase for PCR. This bacterium is thermophilic, which means it thrives in hot settings.
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संबंधित प्रश्न
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
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Downstream processing
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
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Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
