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प्रश्न
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
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उत्तर
Flowchart showing formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Describe briefly the following:
Bioreactors
Explain briefly:
Chitinase
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
In PCR, primers are used for ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
