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प्रश्न
An antibiotic resistance gene in a vector usually helps in the selection of ______.
पर्याय
Competent bacterial cells
Transformed bacterial cells
Recombinant bacterial cells
None of the above
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उत्तर
An antibiotic resistance gene in a vector usually helps in the selection of transformed bacterial cells.
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संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Explain briefly:
Chitinase
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
Gene Amplification using primers can be done by ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
In PCR, primers are used for ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
What is the primary definition of recombinant DNA technology?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
