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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below. - Biology

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प्रश्न

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

आकृती
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उत्तर

A - Denaturation

B - Annealing

C - Extension of primers

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पाठ 11: Biotechnology : Principles and Processes - SHORT ANSWER [पृष्ठ ८०]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 11. | पृष्ठ ८०

संबंधित प्रश्‍न

Name two commonly used bioreactors.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Describe briefly the following:

Bioreactors


Explain briefly:

PCR


Which of the following term used for defining a viral genome incorporated into host DNA?


PCR and restriction fragment length polymorphism are the methods for ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


The first step in recombinant DNA technology is


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


In PCR, primers are used for ______.


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

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