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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

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प्रश्न

Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

आकृती
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उत्तर

A - Denaturation

B - Annealing

C - Extension of primers

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पाठ 11: Biotechnology : Principles and Processes - SHORT ANSWER [पृष्ठ ८०]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
SHORT ANSWER | Q 11. | पृष्ठ ८०

संबंधित प्रश्‍न

Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Explain briefly:

PCR


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


The first step in recombinant DNA technology is


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.


Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?


Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?


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