Advertisements
Advertisements
प्रश्न
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Advertisements
उत्तर
The laboratory uses shake flasks to cultivate and mix materials on a small scale. Microbes, plants, and animal cells, as well as their enzymes, use bioreactors to convert basic materials into specialised products. Bioreactors are used to produce biomass or cell products on a large scale in an aseptic environment. Here, huge volumes of culture (100 to 1000 litres) can be treated. A bioreactor provides the optimal conditions for producing the desired product (temperature, pH, substrate, salts, vitamins, and oxygen). The most commonly used bioreactors are of the stirring type. A bioreactor is a better option than shake flasks. It has an agitator system to mix the contents correctly, an oxygen delivery system to ensure oxygen availability, a foam control system, a temperature control system, a pH control system, and a sampling port to withdraw small volumes of the culture regularly.
APPEARS IN
संबंधित प्रश्न
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Describe briefly the following:
Downstream processing
State the importance of using a bioreactor.
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
In PCR, primers are used for ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
How is copy number of the plasmid vector related to yield of recombinant protein?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
State the advantage of using Thermostable DNA polymerase.
Which of the following statements is incorrect?
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
What is the primary definition of recombinant DNA technology?
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
Which series of operations is collectively referred to as 'downstream processing'?
