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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

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प्रश्न

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

सविस्तर उत्तर
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उत्तर

The laboratory uses shake flasks to cultivate and mix materials on a small scale. Microbes, plants, and animal cells, as well as their enzymes, use bioreactors to convert basic materials into specialised products. Bioreactors are used to produce biomass or cell products on a large scale in an aseptic environment. Here, huge volumes of culture (100 to 1000 litres) can be treated. A bioreactor provides the optimal conditions for producing the desired product (temperature, pH, substrate, salts, vitamins, and oxygen). The most commonly used bioreactors are of the stirring type. A bioreactor is a better option than shake flasks. It has an agitator system to mix the contents correctly, an oxygen delivery system to ensure oxygen availability, a foam control system, a temperature control system, a pH control system, and a sampling port to withdraw small volumes of the culture regularly.

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पाठ 9: Biotechnology : Principles and Processes - EXERCISES [पृष्ठ १७५]

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एनसीईआरटी Biology [English] Class 12
पाठ 9 Biotechnology : Principles and Processes
EXERCISES | Q 6. | पृष्ठ १७५
नूतन Biology [English] Class 12 ISC
पाठ 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 5. | पृष्ठ ५३५

संबंधित प्रश्‍न

Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Bioreactors


Describe briefly the following:

Downstream processing


Explain briefly:

PCR


Explain briefly:

Chitinase


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


______ plays an important role in blood clotting.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


In PCR, primers are used for ______.


Rising of dough is due to ______.


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

State the advantage of using Thermostable DNA polymerase.


Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


What is the primary definition of recombinant DNA technology?


In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?


What is the process of cutting out and recovering a separated DNA band from an agarose gel called?


Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?


Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?


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