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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Can you recall meiosis and indicate at what stage recombinant DNA is made?

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प्रश्न

Can you recall meiosis and indicate at what stage recombinant DNA is made?

अति संक्षिप्त उत्तर
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उत्तर

Recombinant DNA is formed due to crossing over between non-sister chromatids of a homologous chromosome. It occurs during the pachytene stage of prophase of meiosis I.

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पाठ 9: Biotechnology : Principles and Processes - EXERCISES [पृष्ठ १७५]

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एनसीईआरटी Biology [English] Class 12
पाठ 9 Biotechnology : Principles and Processes
EXERCISES | Q 8. | पृष्ठ १७५
नूतन Biology [English] Class 12 ISC
पाठ 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 7. | पृष्ठ ५३५

संबंधित प्रश्‍न

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Describe briefly the following:

Bioreactors


Describe briefly the following:

Downstream processing


Explain briefly:

Chitinase


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


Which of the following term used for defining a viral genome incorporated into host DNA?


______ plays an important role in blood clotting.


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


State the advantage of using Thermostable DNA polymerase.


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.

  1. Insertion of recombinant DNA into the host cell.
  2. Cutting of DNA at specific location by restriction enzyme.
  3. Isolation of desired DNA fragment.
  4. Amplification of gene of interest using PCR. 

Choose the correct answer from the options given below:


Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.


What is the primary definition of recombinant DNA technology?


Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?


How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?


Which series of operations is collectively referred to as 'downstream processing'?


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