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प्रश्न
How is copy number of the plasmid vector related to yield of recombinant protein?
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उत्तर
Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.
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संबंधित प्रश्न
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Explain briefly:
Chitinase
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Gene Amplification using primers can be done by ______.
In PCR, primers are used for ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
