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How is copy number of the plasmid vector related to yield of recombinant protein? - Biology

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प्रश्न

How is copy number of the plasmid vector related to yield of recombinant protein?

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उत्तर

Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.

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पाठ 11: Biotechnology : Principles and Processes - VERY SHORT ANSWER [पृष्ठ ७८]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
VERY SHORT ANSWER | Q 1. | पृष्ठ ७८

संबंधित प्रश्‍न

Name two commonly used bioreactors.


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Bioreactors


Explain briefly:

Chitinase


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


PCR and restriction fragment length polymorphism are the methods for ______.


Gene Amplification using primers can be done by ______.


The first step in recombinant DNA technology is


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Which of the following statements is incorrect?


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