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प्रश्न
How is copy number of the plasmid vector related to yield of recombinant protein?
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उत्तर
Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.
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संबंधित प्रश्न
Explain briefly:
Chitinase
State the importance of using a bioreactor.
Which of the following term used for defining a viral genome incorporated into host DNA?
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
Rising of dough is due to ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
State the advantage of using Thermostable DNA polymerase.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
What is the primary definition of recombinant DNA technology?
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
Which series of operations is collectively referred to as 'downstream processing'?
