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प्रश्न
How is copy number of the plasmid vector related to yield of recombinant protein?
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उत्तर
Higher the copy number of vector plasmid, higher the copy number of gene and consequently, protein coded by the gene is produced in high amount.
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Explain briefly:
PCR
State the importance of using a bioreactor.
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
PCR and restriction fragment length polymorphism are the methods for ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Rising of dough is due to ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?
