Advertisements
Advertisements
प्रश्न
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
विकल्प
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
Advertisements
उत्तर
A continuous culture system
APPEARS IN
संबंधित प्रश्न
Describe briefly the following:
Bioreactors
Explain briefly:
Chitinase
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
In PCR, primers are used for ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Rising of dough is due to ______.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?
How are bacterial cells typically made competent to facilitate the uptake of recombinant DNA during transformation?
