Advertisements
Advertisements
प्रश्न
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
विकल्प
Laboratory flask of largest capacity
A stirred-tank bioreactor without in-lets and out-lets
A continuous culture system
Any of the above
Advertisements
उत्तर
A continuous culture system
APPEARS IN
संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Describe briefly the following:
Bioreactors
Describe the process of amplification of the “gene of interest” using the PCR technique.
______ plays an important role in blood clotting.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
In PCR, primers are used for ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
What is the primary definition of recombinant DNA technology?
What is the process of cutting out and recovering a separated DNA band from an agarose gel called?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
