Advertisements
Advertisements
प्रश्न
Explain briefly:
Chitinase
Advertisements
उत्तर
Chitinase is a class of enzymes used for the degradation of chitin, which forms a significant component of the fungal cell wall. Therefore, to isolate the DNA enclosed within the cell membrane of the fungus, the enzyme chitinase is used to break the cell to release its genetic material.
APPEARS IN
संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Downstream processing
State the importance of using a bioreactor.
Describe the process of amplification of the “gene of interest” using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
______ plays an important role in blood clotting.
Gene Amplification using primers can be done by ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
