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प्रश्न
Explain briefly:
Chitinase
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उत्तर
Chitinase is a class of enzymes used for the degradation of chitin, which forms a significant component of the fungal cell wall. Therefore, to isolate the DNA enclosed within the cell membrane of the fungus, the enzyme chitinase is used to break the cell to release its genetic material.
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Explain briefly:
PCR
Which of the following term used for defining a viral genome incorporated into host DNA?
______ plays an important role in blood clotting.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
State the advantage of using Thermostable DNA polymerase.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
In agarose gel electrophoresis, why do DNA fragments move toward the positive electrode?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is utilized in Polymerase Chain Reaction (PCR) because it remains active at high temperatures?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
