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प्रश्न
Explain briefly:
Chitinase
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उत्तर
Chitinase is a class of enzymes used for the degradation of chitin, which forms a significant component of the fungal cell wall. Therefore, to isolate the DNA enclosed within the cell membrane of the fungus, the enzyme chitinase is used to break the cell to release its genetic material.
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संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Describe briefly the following:
Bioreactors
Explain briefly:
PCR
State the importance of using a bioreactor.
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
______ plays an important role in blood clotting.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?
In PCR, primers are used for ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Rising of dough is due to ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
How is copy number of the plasmid vector related to yield of recombinant protein?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Which of the following statements is incorrect?
