Advertisements
Advertisements
प्रश्न
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Advertisements
उत्तर
The laboratory uses shake flasks to cultivate and mix materials on a small scale. Microbes, plants, and animal cells, as well as their enzymes, use bioreactors to convert basic materials into specialised products. Bioreactors are used to produce biomass or cell products on a large scale in an aseptic environment. Here, huge volumes of culture (100 to 1000 litres) can be treated. A bioreactor provides the optimal conditions for producing the desired product (temperature, pH, substrate, salts, vitamins, and oxygen). The most commonly used bioreactors are of the stirring type. A bioreactor is a better option than shake flasks. It has an agitator system to mix the contents correctly, an oxygen delivery system to ensure oxygen availability, a foam control system, a temperature control system, a pH control system, and a sampling port to withdraw small volumes of the culture regularly.
APPEARS IN
संबंधित प्रश्न
Name two commonly used bioreactors.
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Explain briefly:
PCR
Explain briefly:
Chitinase
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR and restriction fragment length polymorphism are the methods for ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
The first step in recombinant DNA technology is
In PCR, primers are used for ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
Rising of dough is due to ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.

Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
State the advantage of using Thermostable DNA polymerase.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.

Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
Which of the following statements is incorrect?
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
