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Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks? - Biology

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प्रश्न

Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?

विस्तार में उत्तर
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उत्तर

The laboratory uses shake flasks to cultivate and mix materials on a small scale. Microbes, plants, and animal cells, as well as their enzymes, use bioreactors to convert basic materials into specialised products. Bioreactors are used to produce biomass or cell products on a large scale in an aseptic environment. Here, huge volumes of culture (100 to 1000 litres) can be treated. A bioreactor provides the optimal conditions for producing the desired product (temperature, pH, substrate, salts, vitamins, and oxygen). The most commonly used bioreactors are of the stirring type. A bioreactor is a better option than shake flasks. It has an agitator system to mix the contents correctly, an oxygen delivery system to ensure oxygen availability, a foam control system, a temperature control system, a pH control system, and a sampling port to withdraw small volumes of the culture regularly.

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अध्याय 13: Principles and Processes of Biotechnology - NCERT EXERCISES WITH ANSWERS [पृष्ठ ५३५]

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नूतन Biology [English] Class 12 ISC
अध्याय 13 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 5. | पृष्ठ ५३५

संबंधित प्रश्न

Name two commonly used bioreactors.


Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe briefly the following:

Bioreactors


Explain briefly:

Chitinase


State the importance of using a bioreactor.


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


Rising of dough is due to ______.


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Significance of 'heat shock' method in bacterial transformation is to facilitate ______.


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


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