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प्रश्न
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
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उत्तर
The laboratory uses shake flasks to cultivate and mix materials on a small scale. Microbes, plants, and animal cells, as well as their enzymes, use bioreactors to convert basic materials into specialised products. Bioreactors are used to produce biomass or cell products on a large scale in an aseptic environment. Here, huge volumes of culture (100 to 1000 litres) can be treated. A bioreactor provides the optimal conditions for producing the desired product (temperature, pH, substrate, salts, vitamins, and oxygen). The most commonly used bioreactors are of the stirring type. A bioreactor is a better option than shake flasks. It has an agitator system to mix the contents correctly, an oxygen delivery system to ensure oxygen availability, a foam control system, a temperature control system, a pH control system, and a sampling port to withdraw small volumes of the culture regularly.
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संबंधित प्रश्न
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| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
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