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प्रश्न
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
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उत्तर
Restriction endonuclease enzyme EcoRl is used in molecular biology to cut the foreign DNA and vector DNA to form overhangs (called sticky ends). These sticky ends then form hydrogen bonds with their complementary counterparts. The segments with the help of DNA ligases are joined to produce recombinant DNA.
संबंधित प्रश्न
Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Explain briefly:
Chitinase
______ plays an important role in blood clotting.
Gene Amplification using primers can be done by ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
In PCR, primers are used for ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
