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प्रश्न
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
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उत्तर
Restriction endonuclease enzyme EcoRl is used in molecular biology to cut the foreign DNA and vector DNA to form overhangs (called sticky ends). These sticky ends then form hydrogen bonds with their complementary counterparts. The segments with the help of DNA ligases are joined to produce recombinant DNA.
संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Can you recall meiosis and indicate at what stage recombinant DNA is made?
Describe briefly the following:
Bioreactors
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
