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प्रश्न
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
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उत्तर
Restriction endonuclease enzyme EcoRl is used in molecular biology to cut the foreign DNA and vector DNA to form overhangs (called sticky ends). These sticky ends then form hydrogen bonds with their complementary counterparts. The segments with the help of DNA ligases are joined to produce recombinant DNA.
संबंधित प्रश्न
Name two commonly used bioreactors.
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
State the advantage of using Thermostable DNA polymerase.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
