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प्रश्न
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
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उत्तर
Restriction endonuclease enzyme EcoRl is used in molecular biology to cut the foreign DNA and vector DNA to form overhangs (called sticky ends). These sticky ends then form hydrogen bonds with their complementary counterparts. The segments with the help of DNA ligases are joined to produce recombinant DNA.
संबंधित प्रश्न
Name two commonly used bioreactors.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Describe briefly the following:
Bioreactors
Which of the following term used for defining a viral genome incorporated into host DNA?
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?
How is copy number of the plasmid vector related to yield of recombinant protein?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
