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प्रश्न
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
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उत्तर
Restriction endonuclease enzyme EcoRl is used in molecular biology to cut the foreign DNA and vector DNA to form overhangs (called sticky ends). These sticky ends then form hydrogen bonds with their complementary counterparts. The segments with the help of DNA ligases are joined to produce recombinant DNA.
संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
The first step in recombinant DNA technology is
In PCR, primers are used for ______.
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?
Which of the following statements is incorrect?
Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?
Which enzymes act as 'molecular scissors' to cut purified DNA precisely at specific target sites?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
