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प्रश्न
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
पर्याय
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
Extension of primer end on the template DNA
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उत्तर
Extension of primer end on the template DNA
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| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
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