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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction? - Biology

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प्रश्न

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

पर्याय

  • Denaturation of template DNA

  • Annealing of primers to template DNA

  • Extension of primer end on the template DNA

  • Extension of primer end on the template DNA

MCQ
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उत्तर

Extension of primer end on the template DNA

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पाठ 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 15. | पृष्ठ ७७

संबंधित प्रश्‍न

Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Describe briefly the following:

Bioreactors


State the importance of using a bioreactor.


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


Describe the process of amplification of the “gene of interest” using the PCR technique.


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


Which of the following term used for defining a viral genome incorporated into host DNA?


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


An antibiotic resistance gene in a vector usually helps in the selection of ______.


How is copy number of the plasmid vector related to yield of recombinant protein?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

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