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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction? - Biology

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प्रश्न

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

पर्याय

  • Denaturation of template DNA

  • Annealing of primers to template DNA

  • Extension of primer end on the template DNA

  • Extension of primer end on the template DNA

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उत्तर

Extension of primer end on the template DNA

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पाठ 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 15. | पृष्ठ ७७

संबंधित प्रश्‍न

Name two commonly used bioreactors.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


______ plays an important role in blood clotting.


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Rising of dough is due to ______.


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


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