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कर्नाटक बोर्ड पी.यू.सी.पीयूसी विज्ञान 2nd PUC Class 12

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

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प्रश्न

Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?

पर्याय

  • Denaturation of template DNA

  • Annealing of primers to template DNA

  • Extension of primer end on the template DNA

  • Extension of primer end on the template DNA

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उत्तर

Extension of primer end on the template DNA

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पाठ 11: Biotechnology : Principles and Processes - MULTIPLE CHOICE QUESTIONS [पृष्ठ ७७]

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एनसीईआरटी एक्झांप्लर Biology Exemplar [English] Class 12
पाठ 11 Biotechnology : Principles and Processes
MULTIPLE CHOICE QUESTIONS | Q 15. | पृष्ठ ७७

संबंधित प्रश्‍न

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


Can you recall meiosis and indicate at what stage recombinant DNA is made?


Describe the process of amplification of the “gene of interest” using the PCR technique.


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


PCR and restriction fragment length polymorphism are the methods for ______.


Gene Amplification using primers can be done by ______.


The first step in recombinant DNA technology is


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

In the provided agarose gel electrophoresis image, what is represented in lane 1 compared to lanes 2 through 4?


Which of the following are direct methods used for introducing recombinant DNA into host cells besides chemical transformation?


Which series of operations is collectively referred to as 'downstream processing'?


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