Advertisements
Advertisements
Question
Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?
Options
Denaturation of template DNA
Annealing of primers to template DNA
Extension of primer end on the template DNA
Extension of primer end on the template DNA
Advertisements
Solution
Extension of primer end on the template DNA
APPEARS IN
RELATED QUESTIONS
Name two commonly used bioreactors.
Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?
Explain briefly:
PCR
State the importance of using a bioreactor.
Describe the process of amplification of the “gene of interest” using the PCR technique.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Which of the following term used for defining a viral genome incorporated into host DNA?
PCR and restriction fragment length polymorphism are the methods for ______.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
Gene Amplification using primers can be done by ______.
Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?
Rising of dough is due to ______.
A bacterial cell was transformed with a recombinant DNA molecule that was generated using a human gene. However, the transformed cells did not produce the desired protein. Reasons could be ______.
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.
Answer the following questions w.r.t. the given paragraph:
- List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
- Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
- Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
