English
Karnataka Board PUCPUC Science 2nd PUC Class 12

Describe briefly the following: Bioreactors

Advertisements
Advertisements

Question

Describe briefly the following:

Bioreactors

Very Long Answer
Advertisements

Solution

Bioreactors can be thought of as vessels in which raw materials are biologically converted into specific products, individual enzymes, etc., using microbial plant, animal, or human cells. A bioreactor provides the optimal conditions for achieving the desired product by providing optimum growth conditions (temperature, pH, substrate, salts, vitamins, oxygen). The most commonly used bioreactor is the stirring type. Stirred-tank reactors are usually cylindrical or have a curved base. This helps mix the material inside the reactor. Stirrers not only mix the material inside the reactor but also ensure the availability of oxygen everywhere in the reactor. Every bioreactor has an agitator system. It also has an oxygen delivery system, foam control system, temperature control system, and pH control device. The reaction is controlled by a system and sampling, which allows small volumes of the cultured product to be periodically removed.

shaalaa.com
  Is there an error in this question or solution?
Chapter 9: Biotechnology : Principles and Processes - EXERCISES [Page 176]

APPEARS IN

NCERT Biology [English] Class 12
Chapter 9 Biotechnology : Principles and Processes
EXERCISES | Q 10. (b) | Page 176
Nootan Biology [English] Class 12 ISC
Chapter 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 9. (ii) | Page 535

RELATED QUESTIONS

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?


Besides better aeration and mixing properties, what other advantages do stirred tank bioreactors have over shake flasks?


State the importance of using a bioreactor.


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Which of the following term used for defining a viral genome incorporated into host DNA?


______ plays an important role in blood clotting.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


The first step in recombinant DNA technology is


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


Rising of dough is due to ______.


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.


Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.

Reason (R): The primers bind to the double stranded DNA at their complementary regions.


Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Identify the part of a bio-reactor which is used as a foam braker from the given figure.


Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Which of the following statements is incorrect?


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×