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Question
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.
Options
Polysaccharides
RNA
DNA
Histones
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Solution
During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out DNA.
Explanation:
The first step in the purification process for recombinant DNA technology is the isolation of genetic material, which is DNA in most organisms. DNA must be isolated in pure form for further use. The cell is lysed to release DNA and other macromolecules, as it is enclosed by membranes. Various enzymes are used for this purpose. DNA is associated with proteins such as histones, which are removed by protease treatment. When chilled ethanol is added, purified DNA precipitates. It is visible as a collection of fine threads, which are further isolated by spooling.
