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Question
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
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Solution
The flow chart shows the three steps involved in the process of PCR showing the following -
- Denaturation The DNA strands are treated with a temperature of 94°C (Heat) and the strands are separated.
- Annealing The primers anneal to the complementary strands
- Extension The DNA polymerase facilitates the extension of the strands.
OR
Polymerase Chain Reaction
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