English
Karnataka Board PUCPUC Science 2nd PUC Class 12

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

Advertisements
Advertisements

Question

From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

Short Answer
Advertisements

Solution

Both DNA (deoxyribonucleic acid) and enzymes are macromolecules. DNA is a polymer of deoxyribonucleotides, and enzymes are proteins; hence, these are polymers of amino acids. But DNA is larger in molecular size than proteins because protein synthesis is regulated by small segments of DNA, called genes, and a single DNA molecule can encode many proteins.

shaalaa.com
  Is there an error in this question or solution?
Chapter 9: Biotechnology : Principles and Processes - EXERCISES [Page 175]

APPEARS IN

NCERT Biology [English] Class 12
Chapter 9 Biotechnology : Principles and Processes
EXERCISES | Q 3. | Page 175
Nootan Biology [English] Class 12 ISC
Chapter 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 3. | Page 535

RELATED QUESTIONS

Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).


Describe briefly the following:

Bioreactors


State the importance of using a bioreactor.


Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.


Which of the following term used for defining a viral genome incorporated into host DNA?


DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


Blood stains are found at the site of a murder. It DNA profiling technique is to be used for identifying the criminals, which of the following is ideal for use?


In addition to the Taq polymerase enzyme, which other thermostable DNA polymerases have been isolated to be used in PCR?


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


During the process of gene amplification using PCR, if a very high temperature is not maintained in the beginning, then which of the following steps of PCR will be affected first?


Rising of dough is due to ______.


Which of the following steps are catalysed by Taq DNA polymerase in a PCR reaction?


Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?


How is copy number of the plasmid vector related to yield of recombinant protein?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Given below are two statements:

Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.

Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.

In the light of the above statements, choose the most appropriate answer from the options given below:


Which of the following statements is incorrect?


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×