English
Karnataka Board PUCPUC Science 2nd PUC Class 12

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Advertisements
Advertisements

Question

Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.

Diagram
Advertisements

Solution

shaalaa.com
  Is there an error in this question or solution?
Chapter 9: Biotechnology : Principles and Processes - EXERCISES [Page 175]

APPEARS IN

NCERT Biology [English] Class 12
Chapter 9 Biotechnology : Principles and Processes
EXERCISES | Q 2. | Page 175
Nootan Biology [English] Class 12 ISC
Chapter 11 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 2. | Page 535

RELATED QUESTIONS

Explain with the help of a suitable example the naming of a restriction endonuclease.


Mention the difference in the mode of action of exonuclease and endonuclease.


How does a restriction nuclease function? Explain


Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.


Explain briefly:

Restriction enzymes and DNA


Explain the roles of the following with the help of an example each in recombinant DNA technology :

Restriction Enzymes


Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.


Give a reason why :
Single cloning site is preferred in a vector.


The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.

Applying Chargaff’s rule, what conclusion can be drawn?


A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.


Restriction enzymes ______.


There is a restriction endonudease called as EcoRI. What does co part in it stands for?


DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______


A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______


'Restriction' in Restriction enzyme refers to ______.


The role of DNA ligase in the construction of a recombinant DNA molecule is ______.


Which of the following bacteria is not a source of restriction endonuclease?


Would you choose an exonuclease while producing a recombinant DNA molecule?


Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.


A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.


How does one visualise DNA on an agarose gel?


Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing 

  1. Anode end
  2. smallest/lightest DNA strand in the matrix
  3. Agarose gel


State the importance of elution in this process.


Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.


Share
Notifications

Englishहिंदीमराठी


      Forgot password?
Use app×