Question

Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?

Options

• They reliably recognise and cut at very specific target sequences

• They indiscriminately degrade all viral nucleic acids inside bacterial cells

• They join blunt-ended DNA fragments more efficiently than DNA ligase

• They synthesize complementary DNA strands without needing a primer

Answer 1

Type II restriction enzymes are the primary molecular scissors utilized in genetic engineering because they reliably recognize and cleave double-stranded DNA at exact, specific target sequences.