Advertisements
Advertisements
Question
Which of the following enzymes catalyse the removal of nucleotides from the ends of DNA?
Options
Endonuclease
Exonuclease
DNA ligase
Hind - II
Advertisements
Solution
Exonuclease
Explanation:
The enzyme that catalyzes the removal of nucleotides from the ends of DNA is exonuclease. Exonuclease specifically removes nucleotides one by one from the ends of a DNA strand, whereas endonucleases cut DNA at internal sites. DNA ligase joins DNA fragments but does not remove nucleotides. Hind II is a type of restriction endonuclease that cuts DNA at specific internal sequences, not at the ends.
RELATED QUESTIONS
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
DNA fragments separate according to size through?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
Which of the following statements does not hold true for restriction enzyme?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
Identify the activity of endonuclease and exonuclease in the given image.
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
