Question

Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.

\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]

\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]

Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.

Options

• 5' C - T - G	C - A - G 3'
  3' G - A - C - G - T	C 5'

• 5' C - T	G - C - A - G 3'
  3' G - A - G - C	T - C 5'

• 5' C - T - G - C	A - G 3'
  3' G - A - C - G	T - C 5'

• 5' C - T - G - C - A	G 3'
  3' G	A - C - G - T - C 5'

Answer 1

5' C - T - G - C - A	G 3'
3' G	A - C - G - T - C 5'

Explanation: 

Enzyme Pst-I makes a staggered cut of the DNA at its recognition sequence.