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Question
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
Options
5' C - T - G C - A - G 3' 3' G - A - C - G - T C 5' 5' C - T G - C - A - G 3' 3' G - A - G - C T - C 5' 5' C - T - G - C A - G 3' 3' G - A - C - G T - C 5' 5' C - T - G - C - A G 3' 3' G A - C - G - T - C 5'
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Solution
| 5' C - T - G - C - A | G 3' |
| 3' G | A - C - G - T - C 5' |
Explanation:
Enzyme Pst-I makes a staggered cut of the DNA at its recognition sequence.
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