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प्रश्न
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
पर्याय
5' C - T - G C - A - G 3' 3' G - A - C - G - T C 5' 5' C - T G - C - A - G 3' 3' G - A - G - C T - C 5' 5' C - T - G - C A - G 3' 3' G - A - C - G T - C 5' 5' C - T - G - C - A G 3' 3' G A - C - G - T - C 5'
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उत्तर
| 5' C - T - G - C - A | G 3' |
| 3' G | A - C - G - T - C 5' |
Explanation:
Enzyme Pst-I makes a staggered cut of the DNA at its recognition sequence.
संबंधित प्रश्न
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
'Restriction' in restriction enzyme refers to
Would you choose an exonuclease while producing a recombinant DNA molecule?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
State the principle involved in separation of DNA fragments using gel electrophoresis.
What is the precise definition of a palindromic sequence in DNA?
How are separated DNA bands visualized following agarose gel electrophoresis?
