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प्रश्न
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
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उत्तर
(I) Restriction endonucleases are named as follows:
1st alphabet represents the genus of the organism from which the enzyme is isolated.
2nd and 3rd alphabet represent the species of the organism.
4th alphabet represents the strain.
The Roman number represents the order of isolation or discovery of the enzyme.
EcoRI comes from the Escherichia coli RYB strain.
In EcoRI, 'E' comes from the genus 'Escherichia' and 'co' comes from the species name 'Coli'.
The letter 'R' is derived from the name of the strain RYB.
Roman numbers following the names indicate the order in which the enzymes were isolated from that strain of bacteria.
(II) The recognition sequence where EcoRI cleaves the DNA molecule is G/AATTC. Such sequences have a complementary sequence, CTTAA/G, which is known as a palindromic sequence.
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संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
