Advertisements
Advertisements
प्रश्न
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Advertisements
उत्तर
(a) No bands will be obtained as all DNA will be seen in the well only; DNA fragments being negatively charged will not move towards the negative end/cathode. DNA being negatively charged will remain stationed at the positive end/anode end of the agar block.
(b)
- The position of the positive terminal/end/anode and the negative terminal/end/cathode was inter-changed.
- The fragment with the least base pairs will get separated faster and move faster to the anode end.
APPEARS IN
संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
While isolating DNA from bacteria, which of the following enzymes is not required?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
Which of the following represents the specific palindromic recognition sequence cleaved by the restriction endonuclease EcoRI?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
