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प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
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उत्तर
Exonuclease removes the nucleotides from the ends of the DNA chain while endonuclease cuts the DNA at the specific positions within the DNA strand.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Restriction enzymes ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Hind II always cuts DNA molecules at a particular point called recognition sequence and it consists of ______.
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
