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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
While isolating DNA from bacteria, which of the following enzymes is not required?
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
