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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Do eukaryotic cells have restriction endonucleases? Justify your answer.
Collect 5 examples of palindromic DNA sequences. Better try to create a palindromic sequence by following base-pair rules.
Give a reason why :
Single cloning site is preferred in a vector.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
'Restriction' in restriction enzyme refers to
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
State the principle involved in separation of DNA fragments using gel electrophoresis.
