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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
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Applying Chargaff’s rule, what conclusion can be drawn?
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
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In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
While isolating DNA from bacteria, which of the following enzymes is not required?
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How does one visualise DNA on an agarose gel?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
