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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
-
Ist letter represents the genus of the organism from which the enzyme is derived.
-
IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
-
Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Distinguish between exonuclease and endonuclease.
Give a reason why :
Single cloning site is preferred in a vector.
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Would you choose an exonuclease while producing a recombinant DNA molecule?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
