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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
-
Ist letter represents the genus of the organism from which the enzyme is derived.
-
IInd and IIIrd letters represent the species of the organism.
-
IVth letter represents the name of the strain.
-
Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
How does a restriction nuclease function? Explain
Explain briefly:
Restriction enzymes and DNA
Distinguish between exonuclease and endonuclease.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
'Restriction' in restriction enzyme refers to
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
How does one visualise DNA on an agarose gel?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel

State the principle involved in separation of DNA fragments using gel electrophoresis.
