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प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
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उत्तर
The naming of a restriction endonuclease is done in the following way:
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Ist letter represents the genus of the organism from which the enzyme is derived.
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IInd and IIIrd letters represent the species of the organism.
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IVth letter represents the name of the strain.
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Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
How does a restriction nuclease function? Explain
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
'Restriction' in Restriction enzyme refers to ______.
Which of the following bacteria is not a source of restriction endonuclease?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is elution?
State the importance of elution in this process.
State the principle involved in separation of DNA fragments using gel electrophoresis.
