Advertisements
Advertisements
प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Advertisements
उत्तर
The naming of a restriction endonuclease is done in the following way:
-
Ist letter represents the genus of the organism from which the enzyme is derived.
-
IInd and IIIrd letters represent the species of the organism.
-
IVth letter represents the name of the strain.
-
Roman number represents order of isolation.
For example, In EcoRI − Derived from E.coli, strain R.
It is the Ist to be discovered.
संबंधित प्रश्न
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Explain briefly:
Restriction enzymes and DNA
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
How does one visualise DNA on an agarose gel?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
