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प्रश्न
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
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उत्तर
It is because plasmid is a circular DNA molecule. When cut with enzyme, it becomes linear but does not get fragmented. Whereas, a linear DNA molecule gets cut into two fragments. Hence, a single DNA band is observed for plasmid while two DNA bands are observed for linear DNA in agarose gel.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Explain briefly:
Restriction enzymes and DNA
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
'Restriction' in Restriction enzyme refers to ______.
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
What is elution?
State the importance of elution in this process.
Which of the following was the very first restriction endonuclease to be isolated and characterized?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
