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प्रश्न
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
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उत्तर
It is because plasmid is a circular DNA molecule. When cut with enzyme, it becomes linear but does not get fragmented. Whereas, a linear DNA molecule gets cut into two fragments. Hence, a single DNA band is observed for plasmid while two DNA bands are observed for linear DNA in agarose gel.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
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Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
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Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
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'Restriction' in Restriction enzyme refers to ______.
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Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
