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प्रश्न
How are DNA fragments visualised once they are separated by gel electrophoresis?
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उत्तर
The separated DNA fragments can be visualised only after staining the DNA with a compound known as ethidium bromide followed by exposure to UV radiation (you cannot see pure DNA fragments in the visible light and without staining). You can see bright orange coloured bands of DNA in a ethidium bromide stained gel exposed to UV light.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
Mention the difference in the mode of action of exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in restriction enzyme refers to
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
'Restriction' in Restriction enzyme refers to ______.
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
State the principle involved in separation of DNA fragments using gel electrophoresis.
Identify the activity of endonuclease and exonuclease in the given image.
