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प्रश्न
While isolating DNA from bacteria, which of the following enzymes is not required?
पर्याय
Lysozyme
Ribonuclease
Deoxyribonuclease
Protease
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उत्तर
Deoxyribonuclease
Explanation:
While isolating DNA from bacteria, deoxyribonuclease is not required because it degrades DNA itself. Ribonuclease, protease, and lysozyme are necessary to remove RNA, proteins, and to break cell walls, respectively.
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संबंधित प्रश्न
Explain with the help of a suitable example the naming of a restriction endonuclease.
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Why is the enzyme cellulase needed for isolating genetic material from plant cells and not form the animal cells?
Mention the difference in the mode of action of exonuclease and endonuclease.
Suggest a technique to a researcher who needs to separate fragments of DNA.
Explain briefly:
Restriction enzymes and DNA
How does restriction endonuclease function?
Which of the following radioisotope is not suitable for DNA labeling based studies?
Restriction enzymes ______.
Molecular scissors, which cut DNA at specific site is ______.
A specific recognition sequence identified by endonucleases to make cuts at specific positions within the DNA is ______
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is elution?
'EcoRI' has played a very significant role in rDNA technology.
- Explain the convention for naming EcoRI.
- Write the recognition site and the cleavage sites of this restriction endonuclease.
How are DNA fragments visualised once they are separated by gel electrophoresis?
In the restriction endonuclease EcoRI, what do the letter 'R' and the Roman numeral 'I' represent?
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
During agarose gel electrophoresis, why do DNA fragments migrate toward the anode (positive electrode)?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
