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प्रश्न
The role of DNA ligase in the construction of a recombinant DNA molecule is ______.
पर्याय
Formation of phosphodiester bond between two DNA fragments
Formation of hydrogen bonds between sticky ends of DNA fragments
Ligation of all purime and pyrimidine bases
None of the above
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उत्तर
The role of DNA ligase in the construction of a recombinant DNA molecule is formation of phosphodiester bond between two DNA fragments.
APPEARS IN
संबंधित प्रश्न
Distinguish between exonuclease and endonuclease.
Answer the following question.
Explain the significance of palindromic nucleotide sequence in the formation of recombinant DNA.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
Molecular scissors, which cut DNA at specific site is ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Which of the following statements does not hold true for restriction enzyme?
Would you choose an exonuclease while producing a recombinant DNA molecule?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
Given below is the stepwise schematic representation of the process of electrophoresis. Identify the 'alphabets' representing
- Anode end
- smallest/lightest DNA strand in the matrix
- Agarose gel
What is elution?
Identify the activity of endonuclease and exonuclease in the given image.
Why is it mandatory to cut both the vector DNA and the foreign source DNA with the exact same restriction enzyme when constructing recombinant DNA?
Why are Type II restriction endonucleases the primary class of restriction enzymes used in genetic manipulation?
