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प्रश्न
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
पर्याय
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
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उत्तर
Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.
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संबंधित प्रश्न
Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.
From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?
Explain briefly:
PCR
State the importance of using a bioreactor.
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
______ plays an important role in blood clotting.
DNA fragments generated by the restriction endonuclease in a chemical reaction can be separated by ______.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
In PCR, primers are used for ______.
Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the following paragraph and answer the questions that follow:
| Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy. |
- Name the human disease for which the gene therapy was used for the first time.
- Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
- Write the possible permanent cure of the disease by the gene therapy that is in progress.
Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
State the advantage of using Thermostable DNA polymerase.
Main steps in the formation of Recombinant DNA are given below. Arrange these steps in a correct sequence.
- Insertion of recombinant DNA into the host cell.
- Cutting of DNA at specific location by restriction enzyme.
- Isolation of desired DNA fragment.
- Amplification of gene of interest using PCR.
Choose the correct answer from the options given below:
Upon exposure to UV radiation, DNA stained with ethidium bromide will show ______.
Which enzyme is specifically used to break open fungal cells during the isolation of genetic material?
Which enzyme is responsible for joining the desired gene to a carrier vector DNA to form recombinant DNA?
