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प्रश्न
Significance of 'heat shock' method in bacterial transformation is to facilitate ______.
पर्याय
Binding of DNA to the cell wall
Uptake of DNA through membrane transport proteins
Uptake of DNA through transient pores in the bacterial cell wall
Expression of antibiotic resistance gene
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उत्तर
Significance of 'heat shock' method in bacterial transformation is to facilitate Uptake of DNA through transient pores in the bacterial cell wall.
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संबंधित प्रश्न
Name two commonly used bioreactors.
Can you list 10 recombinant proteins which are used in medical practice? Find out where they are used as therapeutics (use the internet).
Describe briefly the following:
Downstream processing
Explain briefly:
PCR
Prepare a flow chart in formation of recombinant DNA by the action of restriction endonuclease enzyme EcoRI.
Answer the following question:
Describe the formation of recombinant DNA by the action of EcoRI.
Describe the process of amplification of the “gene of interest” using the PCR technique.
A single strand of nucleic acid tagged with a radioactive molecule is called ______.
PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.
Rising of dough is due to ______.
An antibiotic resistance gene in a vector usually helps in the selection of ______.
How is copy number of the plasmid vector related to yield of recombinant protein?
While doing a PCR, ‘denaturation’ step is missed. What will be its effect on the process?
Identify and explain steps ‘A’, ‘B’ and ‘C’ in the PCR diagram given below.
Illustrate the design of a bioreactor. Highlight the difference between a flask in your laboratory and a bioreactor which allows cells to grow in a continuous culture system.
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
State the advantage of using Thermostable DNA polymerase.
