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From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know? - Biology

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From what you have learnt, can you tell whether enzymes are bigger or DNA is bigger in molecular size? How did you know?

सविस्तर उत्तर
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उत्तर

Enzymes are proteins. Protein molecules are macromolecules with highly complex structures. They are made up of amino acids. Around 300 types of amino acids are found in nature, but only 20 of these amino acids are found in animal and plant cells. Amino acids form chains and are linked to each other by peptide bonds. The sequence of amino acids in the polypeptide chain of each protein molecule is of a specific type. Proteins have very high molecular weight. Proteins made from different amino acids are of different types. Around 50,000 types of proteins are found in our body.

The biological macromolecules of DNA have a complex structure. These are biological macromolecules even larger than proteins (enzymes). Their molecular weight ranges from 106 to 109 Daltons. The DNA molecule is made up of a polynucleotide chain. mRNA, tRNA and rRNA, having a molecular weight less than DNA, are formed. RNA plays an important role in protein synthesis. For RNA synthesis, the DNA molecule gets duplicated at different places and forms small complementary chains, i.e., small molecules of ribonucleotide acid. These are called primers. RNA: The synthesis of inserters is catalysed by the enzyme RNA polymerase. RNA molecules are used for protein synthesis. This feature makes it clear that DNA molecules are larger molecules than proteins (enzymes).

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पाठ 13: Principles and Processes of Biotechnology - NCERT EXERCISES WITH ANSWERS [पृष्ठ ५३५]

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नूतन Biology [English] Class 12 ISC
पाठ 13 Principles and Processes of Biotechnology
NCERT EXERCISES WITH ANSWERS | Q 3. | पृष्ठ ५३५

संबंधित प्रश्‍न

Suggest and describe a technique to obtain multiple copies of a gene of interest in vitro.


Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.


Explain briefly:

Chitinase


State the importance of using a bioreactor.


In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.


Which of the following term used for defining a viral genome incorporated into host DNA?


______ plays an important role in blood clotting.


PCR and restriction fragment length polymorphism are the methods for ______.


A single strand of nucleic acid tagged with a radioactive molecule is called ______.


Gene Amplification using primers can be done by ______.


PCR proceeds mthree distinct steps governed by temperature, they are in order of ______.


The first step in recombinant DNA technology is


In PCR, primers are used for ______.


During the purification process for recombinant DNA technology, the addition of chilled ethanol precipitates out ______.


Rising of dough is due to ______.


Which of the following contributed in popularising the PCR (polymerase chain reactions) technique?


An antibiotic resistance gene in a vector usually helps in the selection of ______.


Which of the following should be chosen for best yield if one were to produce a recombinant protein in large amounts?


Do biomolecules (DNA, protein) exhibit biological activity in anhydrous conditions?


Read the paragraph given below and answer and questions that follow:

Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique.
  1. Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
  2. What is a primer and its importance in PCR?
  3. Write the importance of PCR as a diagnostic tool.

Read the following paragraph and answer the questions that follow:

Biotechnology revolves around the "gene of interest", with an objective to open various avenues for human welfare in health, medicine, pharma, agriculture etc. using different techniques, tools and processes. One of the breakthroughs of biotechnology in medicine is the gene therapy.
  1. Name the human disease for which the gene therapy was used for the first time.
  2. Explain the steps of gene therapy carried to cure the disease using the lymphocytes of the patient. Why is this therapy not a permanent cure of the disease?
  3. Write the possible permanent cure of the disease by the gene therapy that is in progress. 

Write the scientific name of the source organism of the thermostable DNA polymerase used in PCR.


State the advantage of using Thermostable DNA polymerase.


Bioreactors are the containment vehicles of any biotechnology-based production process. For large scale production and for economic reasons the final success of biotechnological process depends on the efficiency of the bioreactor.

Answer the following questions w.r.t. the given paragraph:

  1. List the operational guidelines that must be adhered to so as to achieve optimisation of the bioreactor system. Enlist any four.
  2. Mention the phase of the growth we refer to in the statement "Optimisation of growth and metabolic activity of the cells".
  3. Is the biological product formed in the bioreactor suitable for the intended use immediate? Give reason in support of your answer.

Which of the following statements is incorrect?


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