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प्रश्न
Read the paragraph given below and answer and questions that follow:
| Enzyme Taq polymerase, is extracted from a eubacterial microorganism Thermus aquaticus from Yellowstone National Park in Montana, USA and isolated by Chien et al. (1976). Taq polymerase successfully replaced the DNA polymerase from E.coli that was being used in PCR earlier and this shift revolutionised the PCR technique. |
- Taq polymerase after its discovery replaced E.coli DNA polymerase in PCR technique. Explain giving reasons why was the need felt for the change?
- What is a primer and its importance in PCR?
- Write the importance of PCR as a diagnostic tool.
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उत्तर
- Taq polymerase was found to be an enzyme that could survive the protein-denaturing temperatures needed for PCR. Thus, it took the place of the DNA polymerase from E. coli that was initially utilised in PCR.
- Primers are required for the polymerase chain reaction because they kick off the DNA polymerase enzyme's activity.
- Because they may frequently make diagnoses before signs of infection appear, PCR tests play a critical role in limiting the spread of infections. They are an accurate and reliable tool for diagnosing many infectious diseases.
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संबंधित प्रश्न
Name two commonly used bioreactors.
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Draw a labelled sketch of sparged-stirred-tank bioreactor. Write its application.
In a pathological lab, a series of steps were undertaken for finding the gene of interest. Describe the steps, or make a flow chart showing the process of amplification of this gene of interest.
Gene Amplification using primers can be done by ______.
The first step in recombinant DNA technology is
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Assertion (A): Synthetic oligonucleotide polymers are used during Annealing in a PCR.
Reason (R): The primers bind to the double stranded DNA at their complementary regions.
Identify the part of a bio-reactor which is used as a foam braker from the given figure.
Given below are two statements:
Statement I: The DNA fragments extracted from gel electrophoresis can be used in construction of recombinant DNA.
Statement II: Smaller size DNA fragments are observed near anode while larger fragments are found near the wells in an agarose gel.
In the light of the above statements, choose the most appropriate answer from the options given below:
