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प्रश्न
Which of the following bacteria is not a source of restriction endonuclease?
पर्याय
Haemophilus influenzae
Escherichia coli
Entamoeba coli
Bacillus amyloliquefaciens
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उत्तर
Entamoeba coli
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संबंधित प्रश्न
How are 'sticky ends' formed on a DNA strand? Why are they so called?
Suggest a technique to a researcher who needs to separate fragments of DNA.
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Make a chart (with diagrammatic representation) showing a restriction enzyme, the substrate DNA on which it acts, the site at which it cuts DNA and the product it produces.
Explain briefly:
Restriction enzymes and DNA
Explain the roles of the following with the help of an example each in recombinant DNA technology :
Restriction Enzymes
Give a reason why :
Single cloning site is preferred in a vector.
The total number of nucleotide sequences of DNA that code for a hormone is 1530. The proportion of different bases in the sequence is found to be Adenine = 34%, Guanine = 19%, Cytosine = 23%, Thymine = 19%.
Applying Chargaff’s rule, what conclusion can be drawn?
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
Restriction enzymes ______.
There is a restriction endonudease called as EcoRI. What does co part in it stands for?
'Restriction' in restriction enzyme refers to
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
Restriction enzymes should not have more than one site of action in the cloning site of a vector. Comment.
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
Carefully observe the given picture. A mixture of DNA with fragments ranging from 200 base pairs to 2500 base pairs was electrophoresed on agarose gel with the following arrangement.
(a) What result will be obtained on staining with ethidium bromide? Explain with reason.
(b) The above setup was modified and a band with 250 base pairs was obtained at X.
What change(s) were made to the previous design to obtain a band at X? Why did the band appear at position X?
Given below is the restriction site of a restriction endonuclease Pst-I and the cleavage sites on a DNA molecule.
\[\ce{5' C - T - G - C - A \overset{\downarrow}{-}{G 3'}}\]
\[\ce{3' G\underset{\uparrow}{-} A - C - G - T - C 5'}\]
Choose the option that gives the correct resultant fragments by the action of the enzyme Pst-I.
What are the protruding and hanging stretches of DNA produced by these restriction enzymes called? Describe their role in the formation of rDNA.
How are DNA fragments visualised once they are separated by gel electrophoresis?
