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प्रश्न
Which of the following bacteria is not a source of restriction endonuclease?
पर्याय
Haemophilus influenzae
Escherichia coli
Entamoeba coli
Bacillus amyloliquefaciens
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उत्तर
Entamoeba coli
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संबंधित प्रश्न
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
How does a restriction nuclease function? Explain
How does restriction endonuclease function?
Give a reason why :
Single cloning site is preferred in a vector.
In agarose gel electrophoresis, DNA molecules are separated on the basis of their ______.
Which of the following statements does not hold true for restriction enzyme?
What does H in’ ‘d’ and ‘III’ refer to in the enzyme Hind III?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
How does one visualise DNA on an agarose gel?
What is elution?
State the importance of elution in this process.
How are DNA fragments visualised once they are separated by gel electrophoresis?
How do exonucleases differ fundamentally from endonucleases in their enzymatic site of action?
In the standard nomenclature convention for restriction endonucleases, what do the second and third letters signify?
What is the origin of agarose, the matrix most commonly used in gel electrophoresis?
How are separated DNA bands visualized following agarose gel electrophoresis?
What is the process called when desired, separated DNA bands are physically cut out from the agarose gel and extracted from the gel piece?
