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प्रश्न
Suggest a technique to a researcher who needs to separate fragments of DNA.
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उत्तर
Gel electrophoresis is a technique that can be used to separate DNA fragments. Being negatively charged, DNA fragments get separated according to their size as they move towards the anode under the influence of an electric field.
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संबंधित प्रश्न
Mention the difference in the mode of action of exonuclease and endonuclease.
Name the enzymes that are used for the isolation of DNA from bacterial and fungal cells for recombinant DNA technology.
How does a restriction nuclease function? Explain
Name and describe the technique that helps in separating the DNA fragments formed by the use of restriction endonuclease
Answer the following question.
Write the use of restriction endonuclease in the formation of recombinant DNA.
The DNA fragment separated on an agarose gel can be visualized by staining with ______.
A mixture containing DNA fragments a, b, c and d, with molecular weights of a + b = c, a > b and d > c was subject to agarose get electrophoresis. This position of these fragments from cathode to anode to anode sides of the gel would be ______.
'Restriction' in restriction enzyme refers to
DNA fragments separate according to size through?
DNA strands on a gel stained with ethidium bromide when viewed under UV radiation, appear as ______
Which of the given statements is correct in the context of visualizing DNA molecules separated by agarose gel electrophoresis?
'Restriction' in Restriction enzyme refers to ______.
Which of the following statements does not hold true for restriction enzyme?
Restriction enzymes that are used in the construction of recombinant DNA are endonucleases which cut the DNA at ‘specific-recognition sequence’. What would be the disadvantage if they do not cut the DNA at specific-recognition sequence?
A plasmid DNA and a linear DNA (both are of the same size) have one site for a restriction endonuclease. When cut and separated on agarose gel electrophoresis, plasmid shows one DNA band while linear DNA shows two fragments. Explain.
How does one visualise DNA on an agarose gel?
CTTAAG
GAATTC
- What are such sequences called? Name the enzyme used that recognizes such nucleotide sequences.
- What is their significance in biotechnology?
What is elution?
